Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 615, eBioscience™

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Product Description

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin. Applications Reported: Rat IgG2a Isotype Control eFluor® 615 has been reported for use in immunohistochemical and immunocytochemical staining. Applications Tested: Rat IgG2a Isotype Control eFluor® 615 has been tested by immunocytochemistry or immunohistochemistry. Use at the same concentration as the experimental antibody. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because