Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 615, eBioscience™

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Product Description

Description: The monoclonal mouse IgG1 kappa immunoglobulin is useful as an isotype control. Applications Reported: Mouse IgG1 K Isotype Control eFluor® 615 has been reported for use in immunocytochemical (ICC) and immunohistochemical (IHC-P) staining. Applications Tested: This Mouse IgG1, K Isotype Control eFluor® 615 has been tested by immunocytochemistry on fixed and permeabilized MDCK cells and by immunohistochemistry (IHC-P) on formalin-fixed paraffin embedded human brain tissue. Use isotype control at the same concentration as experimental antibody. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody sig