Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 615, eBioscience™

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Product Description

Description: This is a monoclonal mouse IgG2a antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This eBM2a antibody has been reported for use in immunohistochemical staining and immunocytochemistry. Applications Tested: This eBM2a antibody has been tested by immunocytochemistry and immunohistochemical staining. Use isotype control at the same concentration as experimental antibody. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal becaus