Phospho-FAK (Tyr397) Polyclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
44624G | 100 µL | 580,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesi
Category | Antibody |
Supplier | ThermoFisher |
Application | ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog. |
Host | Rb |
Genebank | PTK2 |
Isotype | IgG |
Crossreactivity | Ck, Drosophila, Hu, Ms, Xenopus |
Clonality | Pab |
Format | Liquid; Dulbecco's PBS; pH 7.3, with 1mg/mL BSA and 0.05% sodium azide |
Working Dilution | Western Blot (1:1,000), Immunohistochemistry (Assay-dependent), Immunocytochemistry (Assay-depend... |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20°C |
Disclaimer | Alternative CatNo: 44-624G |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |