Phospho-FAK (Tyr397) Monoclonal Antibody (141-9)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
44625G | 10 blots | 586,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody will cross-react with the corresponding autophosphorylation site on Proline-rich/Ca2+- activated tyrosine kinase (Pyk2), [pY402]. FAK [pY397] polyclonal antibody (Cat. No. 44-624G) does not cross-react with Pyk2. The antibody has been used in western blotting. Previous lots of this antibody have been used in immunocytochemistry. For western blotting applications, we recommend using the antibody at a 1:1,000 starting dilution. For immunofluorecence staining applications, we recommend a 1:50 starting dilution. Positive control used: Primary chicken embryo fibroblasts expressing human FAK and plated on fibronectin. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20° C. For shipment or short-term storage (up to one week), 2-8° C is sufficient. Expires one year from date of receipt when stored as instructed. Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and
Category | Antibody |
Supplier | ThermoFisher |
Application | ICC, WB |
Regulatory Status | RUO |
Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog. |
Host | Rb |
Clone Number | [141-9] |
Genebank | PTK2 |
Isotype | IgG |
Crossreactivity | Ck, Drosophila, Hu, Ms, Rt, Xenopus |
Clonality | Mab |
Format | Liquid; Dulbecco's PBS; pH 7.3, with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide |
Concentration | 0.5 mg/mL |
Working Dilution | Immunocytochemistry (Assay-dependent), Western Blot (Assay-dependent) |
Purity | Antigen affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20°C |
Disclaimer | Alternative CatNo: 44-625G |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |