Phospho-ERK1/ERK2 (Thr185, Tyr187) Polyclonal Antibody, Alexa Fluor™ 488
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
44680A1 | 100 µL | 598,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
Category | Antibody |
Supplier | ThermoFisher |
Application | ICC |
Regulatory Status | RUO |
Immunogen | Synthetic phosphopeptide derived from the region of ERK1 that contains threonine 202 (corresponding to 185 in ERK2) and tyrosine 204 (corresponding to 187 in ERK2) |
Host | Rb |
Genebank | MAPK1 |
Isotype | IgG |
Crossreactivity | Hu, Ms, Rt |
Clonality | Pab |
Format | Liquid; Dulbecco's PBS; pH 7.3, with 0.2mg/mL BSA and 0.05% sodium azide |
Working Dilution | Immunocytochemistry (Assay-dependent) |
Purity | Antigen affinity chromatography |
Conjugation | Alexa Fluor 488 |
Storage Conditions | -20°C |
Disclaimer | Alternative CatNo: 44-680A1 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |