AIRE Monoclonal Antibody (5H12), eFluor™ 660, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
50-5934-82 | 100 µg | 656,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE). This 58-kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). However, expression of AIRE protein in the periphery remains unclear despite the detection of AIRE mRNA in the lymph node, lymphoid stromal cells, monocytes, and dendritic cells. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation. The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2. Applications Reported: This 5H12 antibody has been reported for use in intracellular flow cytometric analysis and immunohistochemical staining of frozen tissue sections. Applications Tested: This 5H12 antibody has been tested by immunohistochemistry on frozen 5-week-old mouse thymus and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, ICC, IHC |
Regulatory Status | RUO |
Host | Rt |
Clone Number | [5H12] |
Genebank | AIRE |
Isotype | IgG2c,k |
Crossreactivity | Ms |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Immunohistochemistry (Frozen) (10 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (Assay-D... |
Purity | Affinity chromatography |
Conjugation | eFluor 660 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 50593482 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |