Rabbit IgG Isotype Control (RbNP15), Alexa Fluor™ 647, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
51-4616-82 | 100 µg | 340,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: This product is a rabbit monoclonal IgG and can be used as a matched isotype control for recombinant rabbit monoclonal antibodies. The RbNP15 IgG was selected as an isotype control following screening for low background on a variety of mouse and human cell types. Applications Tested: This rabbit monoclonal isotype control has been tested for use as a negative control for flow cytometric analysis. Please use at the same concentration as the experimental antibody. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shift
Category | Antibody |
Supplier | ThermoFisher |
Application | Isotype control, FC |
Regulatory Status | RUO |
Host | Rb |
Clone Number | [RbNP15] |
Crossreactivity | Not Applicable |
Clonality | Recombi |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Control (Assay-dependent), Flow Cytometry (Assay-dependent) |
Purity | Protein A |
Conjugation | Alexa Fluor 647 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 51461682 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |