Staphylococcus aureus Cas9 Monoclonal Antibody (6H4), Alexa Fluor™ 647, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
51-6499-80 | 25 µg | 226,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: This 6H4 monoclonal antibody recognizes Staphylococcus aureus Cas9 endonuclease. Applications Reported: This 6H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 6H4 antibody has been tested by intracellular staining followed by flow cytometric analysis of Staphylococcus aureus derived Cas9-GFP transfected 293T cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at www.thermofisher.com/flowprotocols. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, ICC |
Regulatory Status | RUO |
Immunogen | His-tagged recombinant Cas-9 from Staphylococcus aureus (maps to N-terminus) |
Host | Ms |
Clone Number | [6H4] |
Isotype | IgG2b,k |
Crossreactivity | Bacteria |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Flow Cytometry (0.06 μg/test), Immunocytochemistry (2.5 μg/ml) |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 647 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 51649980 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |