CD11b Monoclonal Antibody (M1/70), Alexa Fluor™ 488, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
53-0112-80 | 25 µg | 137,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions. Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse splenocytes or bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The M1/70 antibody has been tested by immunohistochemistry of frozen tissue and can be used at less than
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, ICC, IHC |
Regulatory Status | RUO |
Host | Rt |
Clone Number | [M1/70] |
Genebank | ITGAM |
Isotype | IgG2b,k |
Crossreactivity | Ms |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.5 mg/mL |
Working Dilution | Immunohistochemistry (Frozen) (10 µg/mL), Flow Cytometry (0.5 µg/test), Immunocytochemistry (10 µ... |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 488 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 53011280 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |