CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), Alexa Fluor™ 488, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
53-1078-42 | 100 Tests | 428,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated, type I transmembrane protein of approximately 105 kDa. It is expressed intracellularly in lysosomal/endosomal membranes in nearly all cells. It is also expressed on the surface of degranulating T cells (to a lesser extent than CD107a) and activated platelets as well as some cancer cells. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease. Applications Reported: This eBioH4B4 (H4B4) antibody has been reported for use in intracellular staining followed by flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay. Applications Tested: This eBioH4B4 (H4B4) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, ICC |
Regulatory Status | RUO |
Host | Ms |
Clone Number | [eBioH4B4 (H4B4)] |
Genebank | LAMP2 |
Isotype | IgG1,k |
Crossreactivity | Hu |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.2% BSA and 0.09% sodium azide |
Concentration | 5 µL/Test |
Working Dilution | Flow Cytometry (5 µL (0.25 µg)/test), Immunocytochemistry (0.25 µg/mL) |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 488 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 53107842 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |