Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

* CHF, excl. 8.1% VAT and shipping costs

Product Description

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin. Applications Reported: The Alexa Fluor® 488 Rat IgG2a Isotype Control has been reported useful as an isotype control for immunofluorescent staining, flow cytometric analysis, immunocytochemistry, and immunohistochemistry. Applications Tested: This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocyte suspension. This can be used at the same concentration as the experimental antibody. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP