FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor™ 488, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
53-4777-42 | 100 Tests | 452,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population. The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque. Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and IHC. Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (St
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, IHC |
Regulatory Status | RUO |
Host | Ms |
Clone Number | [236A/E7] |
Genebank | FOXP3 |
Isotype | IgG1,k |
Crossreactivity | Hu, Primate, Rhesus Monkey |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.2% BSA and 0.09% sodium azide |
Concentration | 5 µL/Test |
Working Dilution | Immunohistochemistry (Paraffin) (Assay-Dependent), Immunohistochemistry (Frozen) (Assay-Dependent... |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 488 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 53477742 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |