Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor™ 488, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
53-5698-82 | 100 µg | 425,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This SolA15 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Refer to Best Protocols for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) protein
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, ICC, IHC |
Regulatory Status | RUO |
Host | Rt |
Clone Number | [SolA15] |
Genebank | MKI67 |
Isotype | IgG2a,k |
Crossreactivity | Dg, Cynomolgus Monkey, Hu, Ms, Primate, Rt |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.5 mg/mL |
Working Dilution | Immunohistochemistry (Paraffin) (1:100), Immunocytochemistry (1:100), Flow Cytometry (0.25 µg/test) |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 488 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 53569882 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |