Actin (muscle) Monoclonal Antibody (HHF35), Alexa Fluor™ 488, eBioscience™

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Product Description

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas. The HHF35 antibody recognizes human, mouse, rat, bovine, canine and non-human primate actin. Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This HHF35 antibody has been tested by immunocytochemistry and can be used at less than or equal to 30 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. The product encoded by this gene belongs to the actin family of proteins, wh