CD86 (B7-2) Monoclonal Antibody (IT2.2), Alexa Fluor™ 700, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
56-0869-42 | 100 Tests | 392,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response. Applications Reported: This IT2.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This IT2.2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Ms |
Clone Number | [IT2.2] |
Genebank | CD86 |
Isotype | IgG2b,k |
Crossreactivity | Hu |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.2% BSA and 0.09% sodium azide |
Concentration | 5 µL/Test |
Working Dilution | Flow Cytometry (5 µL (0.5 µg)/test) |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 700 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 56086942 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |