HELIOS Monoclonal Antibody (22F6), Alexa Fluor™ 700, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
56-9883-42 | 100 Tests | 475,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia. 22F6 has been reported to cross react with rat Helios. Applications Reported: This 22F6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 22F6 antibody has been pre-diluted and tested by flow cytometric analysis of mouse lymph node cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor 700 emits at 723 nm and can be excited with th
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Hm |
Clone Number | [22F6] |
Genebank | IKZF2 |
Isotype | IgG |
Crossreactivity | Dg, Hu, Ms |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.2% BSA and 0.09% sodium azide |
Concentration | 5 µL/Test |
Working Dilution | Flow Cytometry (5 µL (0.25 µg)/test) |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 700 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 56988342 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |