CD163 Monoclonal Antibody (TNKUPJ), Alexa Fluor™ 532, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
58-1631-82 | 100 µg | 483,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: This TNKUPJ monoclonal antibody recognizes mouse CD163. CD163 is a 130 kDa surface receptor expressed by certain subsets of tissue macrophages, including splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages and a small fraction of peritoneal macrophages. In contrast to human blood monocytes, mouse monocytes do not express CD163. Also, unlike human CD163, mouse CD163 is not as readily induced by M2 polarizing cytokines, and it is not a good marker of M2 macrophages. No common cell lines of monocytic or macrophage origin express mouse CD163. In humans, CD163 has been shown to be proteolytically cleaved and shed from the cell surface, and it acts as a soluble anti-inflammatory factor. This TNKUPJ antibody will detect CD163 on fixed and permeabilized cells allowing for staining of the intracellular pool of this receptor. Although CD163 is relatively stable to collagenase digestion, aggressive tissue dissociation protocols might potentially decrease the amount of surface CD163. In these cases intracellular detection is recommended. Applications Reported: This TNKUPJ antibody has been reported for use in flow cytometric analysis. Applications Tested: This TNKUPJ antibody has been tested by flow cytometric analysis of mouse spl
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Rt |
Clone Number | [TNKUPJ] |
Genebank | CD163 |
Isotype | IgG2a,k |
Crossreactivity | Ms |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Flow Cytometry (0.5 µg/test) |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 532 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 58163182 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |