FOXP3 Monoclonal Antibody (FJK-16s), Alexa Fluor™ 532, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
58-5773-80 | 25 µg | 175,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human. Please note that FJK-16s has been optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (cat. 00-5523). The use of other fixation and staining buffers is not recommended. Applications Reported: This FJK-1
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Rt |
Clone Number | [FJK-16s] |
Genebank | FOXP3 |
Isotype | IgG2a,k |
Crossreactivity | Bo, Dg, Ca, Ms, Pg, Rt |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Flow Cytometry (0.5 µg/test) |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 532 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 58577380 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |