CD3 Monoclonal Antibody (UCHT1), Alexa Fluor™ 660, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
606-0038-42 | 100 Tests | 415,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: This UCHT1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UCHT1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700. Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cy
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Ms |
Clone Number | [UCHT1] |
Genebank | CD247 |
Isotype | IgG1,k |
Crossreactivity | Hu |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.2% BSA and 0.09% sodium azide |
Concentration | 5 µL/Test |
Working Dilution | Flow Cytometry (5 µL (0.25 µg)/test) |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 660 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 606003842 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |