T-bet Monoclonal Antibody (eBio4B10 (4B10)), Alexa Fluor™ 660, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
606-5825-82 | 100 µg | 469,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The eBio4B10 (4B10) monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells. Applications Reported: This eBio4B10 (4B10) antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBio4B10 (4B10) antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracel
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Ms |
Clone Number | [eBio4B10 (4B10)] |
Genebank | TBX21 |
Isotype | IgG1,k |
Crossreactivity | Hu, Ms, Rhesus Monkey |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Flow Cytometry (0.25 µg/test) |
Purity | Affinity chromatography |
Conjugation | Alexa Fluor 660 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 606582582 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |