Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright™ 645, eBioscience™

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Product Description

Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin. Applications Reported: Mouse IgG2b Isotype Control Super Bright 645 has been reported for use in flow cytometric analysis. Applications Tested: Mouse IgG2b Isotype Control Super Bright 645 has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5