Carboxypeptidase Y Monoclonal Antibody (10A5B5)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
A6428 | 250 µg | 778,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Antibody binding specificity was determined by Particle Concentration Fluorescence Immunoassay (PCFIA) using the yeast caboxypeptidase, by Western blot immunoassay using protein extracts from yeast, and by indirect immunofluorescence of fixed yeast cells.The antibody can be used to detect the yeast carboxypeptidase on Western blots or to immunolocalize the carboxypeptidase in fixed yeast cells. The antibody is ideal for screening for caroboxypeptidase secretion (Vps-phenotype) in yeast using the colony immunoblot overlay assay. The antibody has been extensively used in screens for new vps mutations, as well as in complementation analysis with the vps mutant collection. Carboxypeptidase Y is Involved in the degradation of small peptides. It digests peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in the P1 position of an ester substrate.
Category | Antibody |
Supplier | ThermoFisher |
Application | ICC, WB |
Regulatory Status | RUO |
Immunogen | Purified yeast CPY (carboxypeptidase) |
Host | Ms |
Clone Number | [10A5B5] |
Genebank | PRC1 |
Isotype | IgG2a,k |
Crossreactivity | Yeast |
Clonality | Mab |
Format | Liquid; HEPES buffered saline; , with 0.02% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Western Blot (0.25 µg/mL), Immunocytochemistry (20 µg/mL) |
Purity | Ig fraction |
Conjugation | Unconjugated |
Storage Conditions | 4° C, do not freeze |
Disclaimer | Alternative CatNo: A-6428 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |