p53 Monoclonal Antibody (DO-1)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
AHO0152 | 500 µL | 600,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody reacts with mutant and wild form p53. It reacts with an epitope at aa 20-25. Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min. Recommended positive controls include SK-BR-3 cells, MDA-231 cells, and colon carcinoma. The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the f
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, ICC, IHC, IP, WB |
Regulatory Status | RUO |
Immunogen | Recombinant human wild type p53 protein expressed in E. coli. |
Host | Ms |
Clone Number | [DO-1] |
Genebank | TP53 |
Isotype | IgG2a |
Crossreactivity | Hu, Rhesus Monkey |
Clonality | Mab |
Format | Liquid; PBS; pH 7.4, with 0.2% BSA and 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Immunoprecipitation (Assay-Dependent), Immunohistochemistry (Paraffin) (Assay-Dependent), Western... |
Purity | Protein A |
Conjugation | Unconjugated |
Storage Conditions | -20°C |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |