p53 Monoclonal Antibody (DO-1)

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Product Description

This antibody reacts with mutant and wild form p53. It reacts with an epitope at aa 20-25. Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min. Recommended positive controls include SK-BR-3 cells, MDA-231 cells, and colon carcinoma. The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the f