ERK2 Monoclonal Antibody (6F8)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA1099 | 100 µg | 598,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
MA1-099 has been successfully used in Western blot, ChIP, flow cytometry, immunofluorescence, immunohistochemistry and immunoprecipitation applications, and reacts with human, dog, rat, mouse and monkey samples. MA1-099 has successfully been used in western blot to detect p42 ERK in HeLa, NRK and MDCK cell lysates. As demonstrated by a Western blot using recombinant ERK1 and ERK2 proteins, MA1-099 can also detect ERK1, but detects ERK2 at much lower concentrations. As demonstrated by a Western blot using a panel of lysates from various species, MA1-099 only detects endogenous ERK2. ERK2 (also known as MAPK1) is a member of the MAP kinase subfamily of Ser/Thr protein kinases. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, FC, ICC, IHC, IP, WB |
Regulatory Status | RUO |
Immunogen | Protein expressed in 293T cells transfected with human MAPK1 expression vector |
Host | Ms |
Clone Number | [6F8] |
Genebank | MAPK1 |
Isotype | IgG2a |
Crossreactivity | Dg, Hu, Ms, Primate, Rt |
Clonality | Mab |
Format | Liquid; PBS; , with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | ChIP Assay (1-3 µL), Immunohistochemistry (Paraffin) (1:100-1:500), Immunocytochemistry (1:200), ... |
Purity | Protein A |
Conjugation | Unconjugated |
Storage Conditions | -20°C |
Disclaimer | Alternative CatNo: MA1-099 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |