TAP Tag Monoclonal Antibody (22F12-1E3), DyLight™ 488

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Product Description

MA1108-D488 detects the Tev cleavage peptide sequence. MA1108-D488 has been successfully used for fluorescent detection of TAP in Western Blot. DyLight 488 has an excitation/emission of 483/518nm. Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.