Phospho-CREB/ATF1 (Ser133, Ser63) Monoclonal Antibody (10E9)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA1114 | 100 µg | 962,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody recognizes CREB protein phosphorylated at serine 133 and ATF1 phosphorylated at serine 63. The antibody was purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography. Aliquot and freeze in liquid nitrogen. Antibody can be stored frozen at -80ºC for up to 1 year. Thaw aliquots at 37ºC; thawed aliquots may be stored at 4ºC for up to 3 months. Avoid repeated freeze/thaw cycles. This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results. Cyclic AMP response element binding protein (CREB) is a basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44/42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, EL, FC, ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | A synthetic phosphopeptide corresponding to amino acids 125-135 surrounding the Ser133 phosphorylation site of human CREB |
Host | Ms |
Clone Number | [10E9] |
Genebank | ATF1 |
Isotype | IgG1 |
Crossreactivity | Dg, Hu, Ms |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with PEG, sucrose, 50% glycerol and 0.09% sodium azide |
Concentration | 0.1 mg/mL |
Working Dilution | Flow Cytometry (1 µg/test), Immunocytochemistry (1:20-1:200), Immunohistochemistry (Paraffin) (1:... |
Purity | Size-exclusion chromatography |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: MA1-114 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |