ERK1 Monoclonal Antibody (12D11)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA113041 | 100 µL | 650,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
MA1-13041 detects ERK1 from human, mouse, and monkey samples. MA1-13041 does not cross react with ERK2. MA1-13041 has been successfully used in Western blot, ChIP, immunofluoresence, and immunohistochemistry procedures. By Western blot, this antibody detects a 44 kDa protein corresponding to ERK1. The MA1-13041 antigen is a recombinant protein corresponding to the full-length sequence of human ERK1. ERK1 is widely expressed and involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and E
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | Full-length human recombinant protein expressed in bacteria. |
Host | Ms |
Clone Number | [12D11] |
Genebank | MAPK3 |
Isotype | IgG |
Crossreactivity | Hu, Ms, Primate |
Clonality | Mab |
Format | Liquid; PBS; , with 1mg/mL BSA and 0.05% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Immunocytochemistry (2 µg/mL), Immunohistochemistry (1:100), ChIP Assay (1-3 µL), Western Blot (1... |
Purity | Protein A |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: MA1-13041 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |