SSEA5 Monoclonal Antibody (8E11), DyLight™ 488


Catalog Number Pack Size List Price* Quantity
MA1144D488 100 µL 528,00 Add

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Product Description

Immunofluorescence and flow cytometric analysis of MA1-144-D488 shows positive staining of SSEA-5 positive pluripotent cells and cultured human embryonal carcinoma NCCIT cells. Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-beta1-3GlcNAc-beta, which is characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells and on the surface of induced pluripotent cells. SSEA-5 was found to proceed the loss of other known stem cell markers and the combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations than the use of other established stem cell markers.

Category Antibody
Supplier ThermoFisher
Application FC, ICC
Regulatory Status RUO
Immunogen Undifferentiated H9 human embryonic stem cells
Host Ms
Clone Number [8E11]
Isotype IgG1,k
Crossreactivity Hu
Clonality Mab
Format Liquid; PBS; , with proprietary stabilizer and 0.02% sodium azide
Concentration 1 mg/mL
Working Dilution Immunocytochemistry (1:10-1:100), Flow Cytometry (1:50-1:500)
Purity Protein A
Conjugation DY 488
Storage Conditions 4° C, do not freeze
Disclaimer Alternative CatNo: MA1-144-D488
Tech. Data Sheet View datasheet
Link To Supplier https://www.thermofisher.com
Material Safety Data Sheet Download PDF