VEGF Monoclonal Antibody (VG76e)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA116626 | 100 µL | 662,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each. For IHC using paraffin sections, microwave treatment with Tris HCl, pH 10 buffer (NOT citrate buffer) is necessary. Suggested positive control: antigen standard for VEGFA (transient overexpression lysate), recombinant human VEGF protein. VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2),
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, ICC, IHC, RIA, WB |
Regulatory Status | RUO |
Immunogen | Human VEGF189 expressed in E.coli. |
Host | Ms |
Clone Number | [VG76e] |
Genebank | VEGFA |
Isotype | IgG1 |
Crossreactivity | Bo, Hu, Sh, Pg, Rt |
Clonality | Mab |
Format | Liquid; PBS; , with 0.02% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Immunohistochemistry (Paraffin) (1:200), Immunocytochemistry (1:50-1:200), Western Blot (0.5-2 µg... |
Purity | Protein G |
Conjugation | Unconjugated |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: MA1-16626 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |