Mouse IgG1 Isotype Control (MOPC-21), DyLight™ 488
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA1191D488 | 100 µg | 485,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
MA1-191-D488 can be used as a negative control in any immunoassay where a DyLight 488-conjugated primary antibody of the mouse IgG1 isotype is used. DyLight 488 has an excitation/emission of 493/518 nm. MA1-191-D488 can be used as isotype control for Dylight 488 conjugated IgG1 mouse monoclonal antibodies in immunofluorescence and flow cytometry analysis. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype
Category | Antibody |
Supplier | ThermoFisher |
Application | Isotype control, FC, ICC, IHC, WB |
Regulatory Status | RUO |
Host | Ms |
Clone Number | [MOPC-21] |
Crossreactivity | Not Applicable |
Format | Liquid; PBS; pH 7.4, with 0.2% BSA and 15mM sodium azide |
Concentration | 0.1 mg/mL |
Working Dilution | Immunohistochemistry (Frozen) (Assay-dependent), Immunohistochemistry (Paraffin) (Assay-dependent... |
Purity | Size-exclusion chromatography |
Conjugation | DY 488 |
Storage Conditions | 4° C, do not freeze |
Disclaimer | Alternative CatNo: MA1-191-D488 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |