NEFM Monoclonal Antibody (NF-09)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA119225 | 100 µg | 480,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody reacts with both the phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species. Immunocytochemistry: Carnoys fixative 2 x 3 min, blocking 1% glycine + 0.2% gelatin 10 min. Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are ver
Category | Antibody |
Supplier | ThermoFisher |
Application | ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | Pellet of pig brain cold stable proteins after depolymerization of microtubules. |
Host | Ms |
Clone Number | [NF-09] |
Genebank | NEFM |
Isotype | IgG2a |
Crossreactivity | Hu, Ma, Ms, Pg, Rt |
Clonality | Mab |
Format | Liquid; PBS; pH 7.4, with 15mM sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Western Blot (1-2 µg/mL), Immunohistochemistry (Paraffin) (Assay-dependent), Immunocytochemistry ... |
Purity | Protein A |
Conjugation | Unconjugated |
Storage Conditions | 4° C, do not freeze |
Disclaimer | Alternative CatNo: MA1-19225 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |