Mouse anti-Bovine Ig (Light chain) Secondary Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA119384 | 100 µg | 270,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Western Blot: Overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM. Anti-Bovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for bovine (cow, ox) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Category | Antibody |
Supplier | ThermoFisher |
Application | Secondary antibody, FC, IHC, WB |
Regulatory Status | RUO |
Immunogen | Fraction of bovine immunoglobulin. |
Host | Ms |
Clone Number | [IVA285-1] |
Crossreactivity | Bo, Sh |
Clonality | Mab |
Format | Liquid; PBS; pH 7.4, with 15mM sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Western Blot (0.5-1 µg/mL), Flow Cytometry (1-4 µg/mL), Immunohistochemistry (Frozen) (Assay-depe... |
Purity | Protein A |
Conjugation | Unconjugated |
Storage Conditions | 4° C, do not freeze |
Disclaimer | Alternative CatNo: MA1-19384 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |