GRK2 Monoclonal Antibody (5D5)

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Product Description

MA1-2013 detects the beta andrenergic receptor kinase 1 in human, mouse and rat samples. MA1-2013 has been successfully used in Western blot, immunocytochemistry, immunofluoresence, immunoprecipitation, and ELISA procedures. By Western blot this antibody detects a ~30 kDa protein representing the beta andrenergeic receptor kinase 1 in human HeLa cells. In immunocytochemistry procedures MA1-2013 recognizes the beta andrenergic receptor kinase 1 in HeLa, Hek-293 and 3T3 cells. Immunofluoresence in HeLa cells yeilds predominantely cytoplasmic membrane associated staining with some staining in the ruffles. The MA1-2013 immunogen is recombinant beta adrenergic receptor kinase 1. G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Sr