Acetylated Lysine Monoclonal Antibody (1C6)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA12021 | 100 µg | 640,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
MA1-2021 detects human and mouse, and monkey acetylated lysine. MA1-2021 has been used successfully in Western blot, immunoprecipitation, dot blot, ChIP Assay, and immunofluorescence (cells) procedures. By Western blot, this antibody may detect a ladder of acetylated proteins in HeLa cell lysate. In immunofluorescence procedures, MA1-2021 recognizes acetylated lysine in gamma irradiated HeLa cells. The MA1-2021 immunogen is a synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3. In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, DB, ICC, IP, WB |
Regulatory Status | RUO |
Immunogen | Synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3. |
Host | Ms |
Clone Number | [1C6] |
Isotype | IgG2b |
Crossreactivity | Chemical |
Clonality | Mab |
Format | Liquid; PBS; , with 1mg/mL BSA and 0.05% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Peptide array (Assay-dependent), Immunoprecipitation (3 µg), Dot Blot (Assay-dependent), Western ... |
Purity | Protein G |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: MA1-2021 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |