DNAI1 Monoclonal Antibody (70.1)

* CHF, excl. 8.1% VAT and shipping costs

Product Description

Recommended positive controls: chicken brain. This antibody is know to detect cytoplasmic Dynein intermediate chains, and reactivity with the axonemal form is not known. By Western blot, MA1-22146 detects a ~ 70 kDa triplet representing the Dynein intermediate chains. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These protein "motors" contain specialized domains that hydrolyze ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kDa), three intermediate chains (70-125 kDa), and at least four light chains (15-25 kDa).