TNFR2 Monoclonal Antibody (22221.311)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA124723 | 100 µg | 590,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Recommended positive controls: human peripheral blood lymphocytes. In Neutralization studies, at 0.5-1.0 µg/mL, this antibody will block 50% of the bioactivity due to 0.3 µg/mL recombinant human sTNF RII, in the presence of 0.25 ng/mL TNF-alpha in a crystal violet cytolytic assay using mouse L929 cells. Anti-Human sTNF RII is tested for its ability to neutralize the biological effect of sTNF-RII but not sTNF RI in a cytolytic assay using mouse L929 cells, in the presence of TNF-alpha. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of the soluble receptor activity on a responsive cell line, when the soluble receptor is present at a concentration just high enough to elicit a maximum response. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, ICC, N |
Regulatory Status | RUO |
Immunogen | Recombinant human sTNF-RII, expressed in E. coli. |
Host | Ms |
Clone Number | [22221.311] |
Genebank | TNFRSF1B |
Isotype | IgG2a |
Crossreactivity | Hu |
Clonality | Mab |
Format | Liquid; PBS; , with no preservative |
Concentration | 0.5 mg/mL |
Working Dilution | Neutralization (0.5-1.0 µg/mL), Flow Cytometry (Assay-dependent), Immunocytochemistry (8-25 µg/mL) |
Purity | purified |
Conjugation | Unconjugated |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: MA1-24723 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |