Cdc27 Monoclonal Antibody (AF3.1)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA124749 | 100 µL | 700,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Recommended positive controls: HeLa nuclear extract. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, ICC, IHC, IP, WB |
Regulatory Status | RUO |
Immunogen | Synthetic peptide, conjugated to KLH, corresponding to residues 814-823 of human Cdc27. |
Host | Ms |
Clone Number | [AF3.1] |
Genebank | CDC27 |
Isotype | IgG2b |
Crossreactivity | Bo, Dg, Hu, Ms, Rt, Xenopus |
Clonality | Mab |
Format | Liquid; 0.01M PBS; pH 7.4, with 15mM sodium azide |
Concentration | 2.3 mg/mL |
Working Dilution | ELISA (Assay-dependent), Western Blot (1-2 µg/mL), Immunohistochemistry (Paraffin) (Assay-depende... |
Purity | Protein A |
Conjugation | Unconjugated |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: MA1-24749 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |