P-Glycoprotein Monoclonal Antibody (C219)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA126528 | 250 µL | 754,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Recommended positive controls: Cell lines or frozen tissue, such as liver or colon. MA1-26528 is expected to cross react with a wide range of mammals due to sequence homology. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. This clone recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, MDR1 P-glycoprotein (~170kDa) and MDR3 P-glycoprotein (~140kDa). In Western blot, boiling samples may reduce visibility of the protein. For flow cytometry, cells should be fixed in 3.7% formaldehyde for 10 min at room temperature to permeabilize the cell membrane. Incubate cells with 5-10 µg antibody for 30-60 min at 4ºC. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, FC, ICC, IHC, IP, WB |
Regulatory Status | RUO |
Immunogen | SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line. |
Host | Ms |
Clone Number | [C219] |
Genebank | ABCB1 |
Isotype | IgG1 |
Crossreactivity | Bo, Fish, Hm, Hu, Ms, Rt, Zebrafish |
Clonality | Mab |
Format | Liquid; PBS; , with 1% BSA and 0.1% sodium azide |
Concentration | 0.72 mg/mL |
Working Dilution | Immunohistochemistry (Frozen) (5-10 µg/mL), Immunohistochemistry (Paraffin) (>1:25), Immunoprecip... |
Purity | purified |
Conjugation | Unconjugated |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: MA1-26528 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |