Mouse IgG1 Isotype Control (W3/25)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA133304 | 250 µg | 1'066,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody is suitable as a negative control for human, bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues tissues. It is not suitable for use as a negative control on rat tissues. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Category | Antibody |
Supplier | ThermoFisher |
Application | Isotype control, FC, IHC |
Regulatory Status | RUO |
Host | Ms |
Clone Number | [W3/25] |
Crossreactivity | Rt |
Format | Liquid; PBS; , with no preservative |
Concentration | 1 mg/mL |
Working Dilution | Immunohistochemistry (Frozen) (Assay-dependent), Flow Cytometry (10 µL for 10^6 cells in 100 µL) |
Purity | Protein A |
Conjugation | Unconjugated |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: MA1-33304 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |