APE1 Monoclonal Antibody (13B 8E5C2)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA1440 | 100 µL | 576,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
MA1-440 detects apurinic/apyrimidinic endonuclease (APE/ref-1) from human, non human primate, canine, mouse and rat samples. MA1-440 has been successfully used in Western blot, immunohistochemical, flow cytometry and immunoprecipitation procedures. By Western blot, this antibody detects a 36 kDa protein representing APE/ref-1 in HeLa cell lysate. Immunohistochemical staining of APE/ref-1 in a variety of normal and cancerous human tissues, including ovaries, cervix, prostate, germ cell tumors, osteosarcomas, and rhabdosarcomas, with MA1-440 yields a staining pattern consistent with nuclear staining. The MA1-440 immunogen is full-length, recombinant human APE protein. Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (ch
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, FC, ICC, IHC, IP, WB |
Regulatory Status | RUO |
Immunogen | Full-length recombinant human APE protein. |
Host | Ms |
Clone Number | [13B 8E5C2] |
Genebank | APEX1 |
Isotype | IgG2a |
Crossreactivity | Dg, Hu, Ms, Primate, Rt |
Clonality | Mab |
Format | Liquid; PBS; , with 0.05% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Immunocytochemistry (1:50-1:500), Western Blot (1:1,000), Immunoprecipitation (5 µg), Immunohisto... |
Purity | purified |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: MA1-440 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |