Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8)

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Product Description

MA1-46093 reacts with DNA-directed RNA Polymerase II Largest Subunit in human, yeast and mouse samples. Expected to cross-react with Drosophilia melanogaster, hamster, S. pombe, and Arabidopsis thaliana due to sequence homology. MA1-46093 has been successfully used in Western Blot, Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, IHC (P) and Immunoprecipitation applications. The MA1-46093 immunogen is 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). This antibody detects both the phosphorylated and non-phosphorylated forms. RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.