NR3C2 Monoclonal Antibody (H10E4C9F)

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Product Description

MA1-620 detects mineralocorticoid receptor (MR) in human, rat, rabbit and chicken tissues. MA1-620 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects a 116 kDa protein representing MR in rat heart homogenate. Immunohistochemical staining of MR in rabbit atrium with MA1-620 results in strong staining of myocytes and endothelial cells. MA1-620 cannot be used for immunoprecipitation. In immunohistochemical studies, staining with MA1-620 is blocked by pre-incubating the sample with aldosterone. MA1-620 can also be used in formalin-fixed, paraffin-embedded sections. Due to the extremely low levels of MR expressed in native tissues, it is recommended that enhanced detection systems be used for Western blotting such as enhanced chemiluminescence. MA1-620 blocks the binding of aldosterone since it is directed against the steroid binding domain of the mineralocorticoid receptor. The antibody can also react with the steroid binding domain for aldosterone on other receptors. The MA1-620 immunogen is aldosterone-3. This antibody was produced using the anti-idiotypic method. Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by bi