HIV1 Tat Monoclonal Antibody (N1)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA171512 | 100 µg | 606,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody reacts with HIV-1 Tat HXB2, BH10, IIIB and HAN strains. It has been mapped to amino acids 71-81 (KQPASQPRGDP – B subtype a.a. consensus). MA1-71512 can be used for chromatin immunoprecipitation (ChIP) analysis of HIV1 Tat. This clone may also be referred to as N1 (Tat N1). HIV-1 Tat ("Trans-Activator of Transcription") consists of 86 to 101 amino acids depending on the subtype. Tat is critical to HIV reproduction by way of compensating for a defect in its genome: the HIV RNA initially has a hairpin-structured portion which prevents full transcription from occurring. However, a small number of RNA transcripts will be made, which allow the Tat protein to be produced. Tat then binds to and phosphorylates cellular factors, eliminating the effect of the hairpin RNA structure and allowing transcription of the HIV RNA. This increases the rate of transcription, providing a positive feedback cycle. The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients.
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, EL, WB |
Regulatory Status | RUO |
Immunogen | Recombinant Tat protein of HIV-1 BH10 purified from E. coli |
Host | Ms |
Clone Number | [N1] |
Isotype | IgG3 |
Crossreactivity | Virus |
Clonality | Mab |
Format | Liquid; PBS; pH 7.4, with 0.1% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Western Blot (2 µg/mL), ELISA (0.08-0.4 µg/mL), ChIP Assay (1-3 µL) |
Purity | Protein G |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: MA1-71512 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |