alpha Tubulin Monoclonal Antibody (YL1/2)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA180017 | 500 µg | 690,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This clone recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody appears to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin). MA1-80017 is predicted to react with amphibia, birds, and echinoderm based on sequence homology. Does not react with nephrotoma suturalis. This antibody is suitable for use as a loading control.The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, ICC, IHC, IP, RIA, WB |
Regulatory Status | RUO |
Immunogen | Yeast tubulin. |
Host | Rt |
Clone Number | [YL1/2] |
Genebank | TUBA1A |
Isotype | IgG2a |
Crossreactivity | Dg, Drosophila, Hu, Ms, Plant, Pg, Rb, Rt, Xenopus, Yeast |
Clonality | Mab |
Format | Liquid; PBS; pH 7.4, with 0.09% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Radioimmune assays (Assay-dependent), Immunoprecipitation (Assay-dependent), Western Blot (2 µg/m... |
Purity | Protein G |
Conjugation | Unconjugated |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: MA1-80017 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |