beta Actin Monoclonal Antibody (AC-15)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA191399 | 100 µL | 636,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Positive control of cultured human or chicken fibroblasts suggested. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, ICC, IHC, WB |
Regulatory Status | RUO |
Immunogen | synthetic peptide DDDIAALVIDNGSGL conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). |
Host | Ms |
Clone Number | [AC-15] |
Genebank | ACTB |
Isotype | IgG1 |
Crossreactivity | Bo, Dg, Ck, Ca, Gp, Hu, Ms, Sh, Pg, Rb, Rt, Xenopus |
Clonality | Mab |
Format | Liquid; Ascites; , with 15mM sodium azide |
Concentration | 3 mg/mL |
Working Dilution | ELISA (Assay-dependent), Western Blot (1:5,000-1:10,000), Immunocytochemistry (1:1000-1:2000), Im... |
Conjugation | Unconjugated |
Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Disclaimer | Alternative CatNo: MA1-91399 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |