HSP70 Monoclonal Antibody (5A5)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA3007 | 250 µL | 698,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
MA3-007 detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, GRP78, and following heat shock, HSP72 from yeast, rat, Drosophila, fish, mouse, avian, amphibian and human samples. MA3-007 has been successfully used in Western blot, immunofluorescence, gel shift, immunohistochemistry (frozen), and immunoprecipitation procedures. By Western blot, this antibody detects proteins from ~70 kDa to ~78 kDa representing different members of the HSP70 family. 2-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. Immunofluorescence staining of HSP70 in heat shocked HeLa cells with MA3-007 results in cytoplasmic staining. The MA3-007 antigen is recombinant human HSP70 over-expressed in E. coli. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 122-264 of human HSP70, a region that has been shown to be involved in ATP binding. This is the first monoclonal antibody reported to react with: 1) the ATP binding region of HSP70, 2) an epitope in the amino terminus of HSP70. HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, i
Category | Antibody |
Supplier | ThermoFisher |
Application | ICC, IHC, IP, WB |
Regulatory Status | RUO |
Immunogen | Human recombinant HSP70 over expressed in E. coli. |
Host | Ms |
Clone Number | [5A5] |
Genebank | HSPA1A |
Isotype | IgG1 |
Crossreactivity | Amphibian, Avian, Dg, Drosophila, Fish, Flatworm, Hu, Ms, Primate, Rt, Yeast |
Clonality | Mab |
Format | Liquid; PBS; , with 1mg/mL BSA and 0.05% sodium azide |
Concentration | 1.0 mg/mL |
Working Dilution | Western Blot (1:250-1:1,000), Immunohistochemistry (Frozen) (Assay-dependent), Gel Shift (Assay-d... |
Purity | Protein A |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: MA3-007 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |