NFATC1 Monoclonal Antibody (7A6)
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
MA3024 | 100 µL | 618,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
MA3-024 detects NFATc1 (NFAT2) from transfected human, rat, mouse, and monkey cells. This antibody does not cross react with NFATc2 (NFAT1). MA3-024 has been successfully used in Western blot, immunoprecipitation, ChIP, immunofluorescence and gel shift procedures. By Western blot, this antibody detects an ~105 kDa band representing transfected NFAT2 from COS cell lysate. The MA3-024 immunogen is bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654. Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell-based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization, including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody, may have to be performed for your high content assay. The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor
Category | Antibody |
Supplier | ThermoFisher |
Application | ChIP, ICC, IHC, IP, WB |
Regulatory Status | RUO |
Immunogen | Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654. |
Host | Ms |
Clone Number | [7A6] |
Genebank | NFATC1 |
Isotype | IgG1 |
Crossreactivity | Hu, Ms, Primate, Rt |
Clonality | Mab |
Format | Liquid; ascites; diluted in PBS, with 0.05% sodium azide |
Working Dilution | Immunocytochemistry (1:250), Western Blot (1:1,000-1:2,000), Gel Shift (Assay-dependent), ChIP As... |
Conjugation | Unconjugated |
Storage Conditions | -20° C, Avoid Freeze/Thaw Cycles |
Disclaimer | Alternative CatNo: MA3-024 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |