mtHSP70 Monoclonal Antibody (JG1)

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Product Description

MA3-028 detects mitochondrial heat shock protein 70 kDa (mtHSP70) from human, non-human primate, canine and mouse tissues. MA3-028 has been successfully used in Western blot, immunocytochemical, immunofluorescence, immunohistochemical (paraffin), and immunoprecipitation procedures. By Western blot, this antibody detects a single ~75 kDa band representing mtHSP70 from U2OS cell homogenate. Immunocytochemical staining of mtHSP70 in DAP.3 cells with MA3-028 results in a worm-like staining pattern, consistent with mitochondrial localization. Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family me