alpha Adaptin Monoclonal Antibody (AC1-M11)

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Product Description

MA3-061 detects alpha-adaptin from bovine, chicken, frog, human, monkey, mouse, pig and rat. This antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons. MA3-061 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody recognizes an ~100 kDa doublet representing the two isoforms of alpha-adaptin from pig brain extract. Immunofluorescence staining of alpha-adaptin in transfected COS cells with MA3-061A yields distinct punctate dots on the plasma membrane. The MA3-061 antigen is purified bovine brain adaptor complexes. Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind d